Figure 6.

Notch1 ablation results in a reduction in GFAP⁺ dormant NSCs in the regenerated SVZ and during aging. A, B, bGal⁺ cells (arrowheads) in the regenerated SVZ 15 d after stopping AraC infusion are reduced in the Notch1-ablated (Notch1^Δ/Δ) compared with control (Notch1^+/+) animals. C, Ablation of Notch1 (Notch1^Δ/Δ; n = 5) before regeneration results in a reduction in bGal⁺GFAP⁺ cells compared with control (Notch1^+/+; n = 5) animals. D, E, BrdU label-retaining cells are present in the regenerated SVZ of control (Notch1^+/+) and Notch1 cKO (Notch1^Δ/Δ) animals and some express GFAP (arrowheads). F, bGal⁺ BrdU-retaining cells are slightly reduced in the regenerated SVZ following deletion of Notch1 (Notch1^Δ/Δ; n = 5) compared with control (Notch1^+/+; n = 5) animals. G, The number of bGal⁺GFAP⁺ BrdU-retaining cells is slightly reduced in the regenerated SVZ of Notch1 cKO (Notch1^Δ/Δ; n = 5) compared with control (Notch1^+/+; n = 5) animals. bGal⁺GFAP⁺ cells in the Notch1 cKO SVZ do not remain mitotically active (PCNA⁺) after regeneration. H, I, In both control and Notch1 cKO animals, GFAP⁺ cells return to a quiescent state (PCNA⁻) in the regenerated SVZ (arrowheads). J, 10 d TAM induction protocol in adult mice followed by 12-month chase (death, arrow). K–M, Ablation of Notch1 in young adult mice (Notch1^Δ/Δ; n = 4) results in a reduction in bGal⁺GFAP⁺ cells when analyzed 1 year later compared with control mice (Notch1^+/+; n = 4). Error bars are SD. Student's t test, *p < 0.05. Scale bars: A, 25 μm; D, E, H, I, K, L, 10 μm.