Figure 6.

Intracellular localization of GluN2B. A, B, Primary cultures of hippocampal neurons were double-stained with anti-GluN2B/synaptophysin (A) or anti-GluN2B/MAP2 (B) antibodies. Transgenically expressed KIF17 (green) was present in TgS/kif17^−/−, TgA/kif17^−/− and TgD/kif17^−/− neurons. Arrows indicate colocalization of GFP-KIF17 and GluN2B in dendritic shafts. Scale bar, 10 μm. C, Quantification of the synaptic density of GluN2B-positive clusters (colabeled with anti-synaptophysin) in dendrites. D, Quantification of the density of GluN2B clusters in dendritic shafts (colabeled with anti-MAP2). E, Comparison of the distribution of GluN2B clusters in dendritic shafts and synapses. Twenty neurons from three animals were examined for each genotype. Data are expressed as mean ± SEM (*p < 0.01; one-way ANOVA and post hoc test).