Figure 8.

Surface expression of endogenous GluN2B and GluN2A in hippocampal neurons. Surface-expressed proteins in cultured neurons were labeled and isolated, and then resolved by SDS-PAGE. A, Immunoblots of levels of GluN2B, GluN2A, and GluR1 in total extracts and surface components. B, Quantification of surface expression experiments. The intensities of GluN2B and GluN2A (total and surface) were normalized to GluR1. Data are expressed as mean ± SEM from three independent experiments. *p < 0.05; one-way ANOVA and post hoc test.