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. 2012 Apr 25;32(17):5757–5771. doi: 10.1523/JNEUROSCI.4231-11.2012

Figure 6.

Figure 6.

Synaptic targets of dA1 interneurons. A, A scheme of the constructs; a synaptic SV2-GFP or synaptobrevin-GFP (SynB) reporter cassette is flanked between two PB arms (PB-LoxP-STOP-LoxP-SV2-GFP-PB; PB-LoxP-STOP-LoxP-SynB-GFP-PB). The integration of the reporter cassette into the genome and the expression of SV2-GFP/SynB-GFP in dA1 neurons is driven by CAG::PBase and Atoh1::Cre. C–K, Sagittal sections of E13.5 cerebellum (C–J″) and medulla/pons (K–K″) from embryos electroporated at E2.5 with the PiggyBac system to label synaptic vesicles of dA1 axons. B, Confocal 3D imaging of SV2-GFP+ synapses in the cerebellum. For all images, each marker is indicated in a different color, higher-magnification views of the boxed areas in the left panels are presented at their respective right panels. C″–J″ represent digital magnifications of boxed areas in C′–J′. Arrowheads indicate SV2-GFP labeled synapses (green), and arrows indicate SV2-GFP/SynB-GFP labeled synapses coexpressing the synaptic markers synaptotagmin/SV2 (yellow). Scale bars are indicated. Cb, cerebellum; DCN, deep cerebellar neurons; EGL, external granular layer; IGL, internal granular layer; Phallo, phalloidin; NL, nuclear laminaris S-Tag, synaptotagmin; Ax-1; axonin 1; Calb, calbindin; A, anterior; P, posterior.