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. 2019 Aug 21;14(8):e0219475. doi: 10.1371/journal.pone.0219475

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© 2019 Bendezu et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) Strategy for cloning ILTV gG from the VFAR-043 strain into pFastBacDual. (B) Western blot detection of secreted ILTV gG from infected Sf9 insect cell supernatants using anti-6x-His tag antibodies. (C) Western blot detection of secreted ILTV gG from infected Sf9 insect cell supernatants using serum from ILTV-infected chicken (Charles Rivers Laboratories). (D) SDS-PAGE detection of purified ILTV gG by Coomassie blue staining. Lane M: Molecular weight marker; Lane 1: ILTV gG recombinant protein. Black arrows indicate the ILTV gG recombinant protein.