Skip to main content
. 2019 Aug 21;14(8):e0219475. doi: 10.1371/journal.pone.0219475

Fig 5. Evaluation of monoclonal antibodies using the ILTV gG recombinant protein in an indirect ELISA assay.

Fig 5

A. Determination of the optimal monoclonal antibody concentration for the detection of ILTV gG. B. Limit of detection for each monoclonal antibody. C. Specificity evaluation for monoclonal antibodies using ILTV gG as a positive control and other related chicken respiratory viruses (Newcastle disease virus [LaSota strain], infectious bronchitis virus [Q1-type strain] and avian metapneumovirus [SHS-FAR strain]). Dashed arrows show the optimal antibody concentration. Cutoff = 0.05. Two-way ANOVA and Tukey’s post hoc were performed. **: p = 0.001. ****: p < 0.0001.