Skip to main content
. 2019 Jul 31;132(15):jcs231241. doi: 10.1242/jcs.231241

Fig. 4.

Fig. 4.

YAP transformation of integrin β4 reporter cells. (A) Expression of Myc–5SA-YAP or empty vector in integrin β4 reporter comma-d1 cells was evaluated by immunoblotting. (B) Expression of the YAP target genes ANKRD1 and Cyr61 by means of qPCR in both populations was quantified and is presented relative to that in empty vector cells (set at 1). (C,D) Soft agar colony (C) and mammosphere formation (D) was assessed in the control and YAP-transformed integrin β4 reporter comma-d1 cells. P1 indicates passage 1 and P2 indicates passage 2. (E) integrin β4 mRNA expression was quantified in the control and YAP-transformed cells by means of qPCR and is presented relative to that in empty vector cells (set at 1). (F) Analysis of integrin β4 surface expression by flow cytometry of integrin β4 reporter cells expressing empty vector or 5SA-YAP. Bar graphs show mean±s.d., with dots representing the results from three independent experiments. *P≤0.05; **P<0.005 (two-tailed t-test).