Glycylation promotes BB cortical attachment and BB-appendage microtubule length. (A) Left panels: Images of longitudinal sections through BBs (Centrin, green) and epiplasm (Epc1, grayscale) in WT and ttll3Δ cells. Right panel, graph showing ttll3Δ cells have more cortically detached BBs at 30°C (WT: n=55, ttll3Δ: n=63). Scale bar: 1 µm. (B) Left panels: Images show BBs (Centrin, green) and BB-appendage microtubules (α-tubulin, red) in WT and ttll3Δ cells at 30°C. Scale bar: 1 μm. Right panel: Graph showing tMTs and pcMTs of mature BBs are shorter in ttll3Δ cells (WT: n=67 cells, 156 tMTs, 194 pcMTs; ttll3Δ: n=75 cells, 141 tMTs, 155 pcMTs).