Fig. 1.

Wnt target gene expression falls into two categories: a ring pattern or a horseshoe pattern. (A) In situ hybridization of Wnt target genes at mid gastrula (stage 11.5). Expression patterns fall into two main categories; ring and horseshoe shape around the blastopore. (B) Schematic of expression pattern for a ring versus horseshoe gene at mid gastrula. (C) qPCR data from eight embryos dissected into dorsal, lateral and ventral marginal zone pieces. eef1a1 was used as an internal control and values were normalized to the ventral pieces. (D) RNA in situ hybridization pattern for wnt8 and fgf8. (E) Activity of Wnt and Fgf signaling pathways at mid-gastrula. RNA in situ hybridization for GFP reporter of Wnt activity is a horseshoe pattern (left). pERK staining for activity of Fgf pathway is in a ring pattern (right). (F) qPCR on same sample as in C for fgf8 and wnt8.