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. 2019 Aug 9;146(15):dev179580. doi: 10.1242/dev.179580

Fig. 6.

Fig. 6.

Ets is sufficient for expression of ring genes in dorsal mesoderm explants but not for expression of horseshoe genes. (A) Schematic of location of dorsal versus lateral/ventral mesoderm in mid-gastrula-stage embryos. (B) Schematic of experiment to make either dorsal or ventral mesoderm from animal cap explants. (C) qPCR from triplicate experiments showing expression of either chordin or brachyury in Activin-treated caps derived from either control, dorsalized (LiCl) or ventralized (UV) embryos. (D) qPCR from triplicate experiments showing expression of either cdx2 or pnhd from dorsal and ventral mesodermal caps with or without the addition of ets2 mRNA. Data are mean±s.d. *P<0.05, Student's t-test. NS, not significant.