Figure 8.

The APL–MB circuit is required post developmentally for reversal learning. A, Heat shock regime: the uninduced group stayed at PT throughout development and adulthood (PT 18°C). The induced group was transferred to RT (30°C) for 3 d to induce transgene expression after the development phase in PT. The induced group was tested in the reversal learning task within 0–6 h after the heat shock treatment. B, The mean reversal training and test PIs were reduced after Gad-RNAi induction in GH146-Gal4. n = 14–20 flies per group. C, After heat shock, the mean reversal training and test PIs were statistically indistinguishable from uninduced controls for both GH146-Gal4/+; Tub-Gal80^ts/+ and Gad-RNAi/+ flies. No significant difference had been found between these two genotypes, so their data were pooled together. n = 20 flies per group. D, Same as A, except that the heat shock lasted for 12 h and flies were transferred into RT when 3 d old. E, The mean reversal training and test PI were reduced after TNTE induction in 17d-Gal4. n = 14 flies per group. Also note that, for unknown reasons, the reversal test PI is negative. F, After heat shock, the mean reversal training and test PI were statistically indistinguishable from uninduced controls for both 17-Gal4/+ and TNTE/+; Tub-Gal80^ts/+ flies. No significant difference had been found between these two genotypes, so their data were pooled together. n = 20 flies per group. Data were shown as means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.