Table 2.
Primary antibodies
| Primary antibody | Company | Reference | Host/isotype | Dilution |
|---|---|---|---|---|
| Anti-KI67 | BD Pharmingen | 556003, clone B56 | Monoclonal mouse IgG1 | 1:200 |
| Anti-caspase3 | Cell Signaling Technology | 9661 | Rabbit polyclonal | 1:500 |
| Anti-pannexin-1 | Diatheva | ANT0027 | Chicken polyclonal | 1:100 |
| Anit-P2X4 | Provided by F. Rassendren, INSERM U661, Montpellier, France | Sim et al. (2006) | Rabbit polyclonal | 1:500 |
| Anti-Mac-2 | American Type Culture Collection | Clone M3/38.10.2.8 HL0.2 | Monoclonal rat IgG2a | 1:1000 |
| Anti-CD68 | Serotec | MCA1957, clone FA-11 | Monoclonal rat IgG2a | 1:400 |
| Anti-IBA1 | Wako | 019-19741 | Rabbit polyclonal | 1:500 |
| Anti-CD11b | Serotec | MCA74GA, clone M1/70.15 | Monoclonal rat IgG2b | 1:400 |
| Anti-β-galactosidase | MP Biomedicals | 55976 | Rabbit polyclonal | 1:500 |