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PF synapses on PCs in cocultures. A–C, Cocultures were double immunostained for calbindin (green) and a PF marker, VGluT1 (red), after 0 (A), 4 (B), and 7 DIV (C). Note that VGluT1 signals are present in PCs throughout coculture period. Scale bar: 20 μm.
