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. 2019 Aug 21;8:e46997. doi: 10.7554/eLife.46997

Figure 8. TLR-mediated stimulation is required for maximal IgM responses to influenza virus infection.

Figure 8.

(A) C57BL/6 (n = 5) and congenic total TLR-deficient mice (n = 5; lacking TLR2, TLR4 and Unc93) were infected with influenza A/Puerto Rico/8/34 for 5 days. Shown are representative FACS plots from control C57BL/6 (top) and TLR-deficient (bottom) mice FACS analysis of MedLN for the presence of B-1 and B-1PC. (B) Number of B-1PC per MedLN as assessed by FACS and (C) number of IgM-secreting cells in MedLN as assessed by ELISPOT analysis. (D-F) Similar analysis as for A-C but using allotype chimeras generated with wild type recipients and B-1 cells from either C57BL/6 or tlr-/- mice. (D) Representative FACS analysis of CD138+ B-1PC pre-gated for live, dump-, B-1 donor (IgMb+) cells in MedLN on days 5 and 7 after influenza infection. (E) Mean ± SD of data summarized from analysis shown in D. (F) Mean ± SD of B-1 IgM-ASC in MedLN on day 7 after infection, as assessed by ELISPOT. Each symbol represents results from one mouse with female mice shown as open symbols, males as closed symbols. Results are combined from two independent experiments. Values were compared using an unpaired Student’s t test (*=p < 0.05, **=p < 0.005, n.s. not significant).