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Mislocalization of exogenous mouse tau in mouse neurons in culture. Cultured mouse neurons from non-Tg mouse hippocampi were transfected at 7 DIV with GFP-tagged mouse tau, the expression of which is regulated by a CMV promoter. They were fixed at 14 DIV and immunostained for total tau (red in the merged image) and MAP2 (in blue). The arrowhead indicates the soma of a neuron that expresses GFP-mouse tau, and the arrow indicates the soma of a nontransfected neuron. Scale bar, 20 μm.
