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. 2019 Aug 14;8:e48311. doi: 10.7554/eLife.48311

Key resources table.

Reagent type
(species) or resource
Designation Source or reference Identifiers Additional
information
Genetic reagent (M. musculus) VGAT-Cre The Jackson laboratory RRID: MGI:5141285 PMID:21745644
Genetic reagent (M. musculus) Orexin KO PMID:10481909 RRID: IMSR_KOMP:VG11744-1.1-Vlcg Dr. Masashi Yanagisawa (University of Texas)
Recombinant DNA reagent AAV.Syn.Flex.GCaMP6s.WPRE.SV40 http://www.addgene.org/100845/ RRID:Addgene_100845 PMID:23868258
Originally purchased from university of Pennsylvania gene
therapy core
Antibody anti-VGAT (Mouse monoclonal) Synaptic System Cat# 131011, RRID:AB_887872 PMID:17444497
(1:500)
Antibody anti-Orexin (Goat polyclonal) Santa Cruz Biotechnology Cat#: SC-8070,
RRID:AB_653610
PMID:16100511
(1:5000)
Software, algorithm SPSS IBM RRID:SCR_002865
Software, algorithm Matlab MathWorks RRID:SCR_001622