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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: Anal Chem. 2019 Jul 12;91(15):9472–9480. doi: 10.1021/acs.analchem.9b00062

Figure 1.

Figure 1.

Native MS analyses using multiple MS instrumentation and subsequent algorithmic deconvolution of a diverse range of pharmaceutically relevant and research grade protein constructs: a) the NIST IgG1k mAb standard analyzed by nESI native-MS mode by Orbitrap-EMR MS; b) an IgG1 lysine-biotin (10 molar biotin equivalent;(34) PNGaseF treated) conjugate, analyzed by nESI native-MS by Q-ToF MS; c) aquaporin-Z analyzed by nESI native-MS by FTICR MS;(35) d) GroEL analyzed by negative native-MS nESI by Q-ToF MS. Insets display the unprocessed data. The most intense charge state, the formylation stoichiometry, and the drug-to-antibody ratio (DAR) values are annotated.