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. Author manuscript; available in PMC: 2020 Aug 20.
Published in final edited form as: Immunity. 2019 Jul 11;51(2):351–366.e6. doi: 10.1016/j.immuni.2019.06.006

Figure 6. Old PCs Regulate Inflammatory Gene Expression in Stromal Cells.

Figure 6.

(A) Representative FACS plot depicting purification strategy of Lin™ CD45™ CD31™ bone marrow stromal cells. Genes differentially expressed by young and old stroma (B) independent of PCs (PC-Ind) and (C) dependent on PCs (PC-Dep). (D) Strategy for purification of BM Ly-6Chigh Ly-6G™ inflammatory monocytes and Ly-6Cint Ly-6G+ granulocytes. Numbers of (E) Ly-6Chigh Ly-6G™ inflammatory monocytes and (F) Ly-6Cint Ly-6G+ granulocytes in BM of young, huCD20 and muCD138 treated old mice. Each symbol represents an individual mouse. Animals used: Young = 4; huCD20 = 8; muCD138 = 6. Statistics: One-way ANOVA with Bonferroni’s correction. Genes differentially expressed by young and old Ly-6Chigh Ly-6G™ inflammatory monocytes (G) independent of PCs (PC-Ind) and (H) dependent on PCs (PC-Dep). Genes differentially expressed by young and old Ly-6Cint Ly-6G+ granulocytes (I) independent of PCs (PC-Ind) and (J) dependent on PCs (PC-Dep). Bars represent average of 2 independent experiments and show average Log2 fold changes in gene expression of samples isolated from huCD20 (orange) and muCD138 (black) treated old mice relative to their young counterparts. Sort purities of stromal cells, monocytes and granulocytes were routinely greater than 99%.