KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Purified CD16/32 (Clone: 93) | Thermo Fisher Scientific | Cat#: 14-0161-86 |
| Unlabeled Mouse IgG | Southern Biotech | Cat#: 0107-01 |
| CD138-Biotin (Clone: 281-2) | BD Biosciences | Cat#: 553713 |
| CD135-Biotin (Clone: A2F10) | Thermo Fisher Scientific | Cat#: 13-1351-85 |
| CD11b-Biotin (Clone: M1/70) | Thermo Fisher Scientific | Cat#: 13-0112-85 |
| Ly-51(BP-1)-Biotin (Clone: 6C3) | BD Biosciences | Cat#: 553159 |
| CD48-FITC (Clone: HM48-1) | BioLegend | Cat#: 103404 |
| CD3ε-FITC (Clone: 145-2C11) | Thermo Fisher Scientific | Cat#: 11-0031-85 |
| CD8α-FITC (Clone: 53-6.7) | Thermo Fisher Scientific | Cat#: 11-0081-85 |
| TCRβ-FITC (Clone: H57-597) | Thermo Fisher Scientific | Cat#: 11-5961-85 |
| TCRγδ-FITC (Clone: UC7-13D5) | Thermo Fisher Scientific | Cat#: 11-5811-85 |
| NK1.1-FITC (Clone: PK136) | Thermo Fisher Scientific | Cat#: 11-5941-85 |
| Gr-1-FITC (Clone: RB6-8C5) | Thermo Fisher Scientific | Cat#: 11-5931-85 |
| Ter-119-FITC (Clone: TER-119) | Thermo Fisher Scientific | Cat#: 11-5921-85 |
| CD45R(B220)-FITC (Clone: RA3-6B2) | Thermo Fisher Scientific | Cat#: 11-0452-85 |
| IgM-FITC (Polyclonal) | Southern Biotech | Cat#: 1020-02 |
| IgA-FITC (Clone: mA-6E1) | Thermo Fisher Scientific | Cat#: 11-4204-83 |
| CD11b-FITC (Clone: M1/70) | Thermo Fisher Scientific | Cat#: 11-0112-85 |
| IgD-FITC (Clone: 11-26) | Southern Biotech | Cat#: 1120-02 |
| CD45-FITC (Clone: 30F-11) | Thermo Fisher Scientific | Cat#: 11-0451-85 |
| Ly-6C-FITC (Clone: AL-21) | BD Biosciences | Cat#: 553104 |
| IgM-PE (Polyclonal) | Southern Biotech | Cat#: 1020-09 |
| IgA-PE (Clone: mA-6E1) | Thermo Fisher Scientific | Cat#: 12-4204-83 |
| Gr-1-PE (Clone: RB6-8C5) | Thermo Fisher Scientific | Cat#: 12-5931-85 |
| CD16/32-PE (Clone: 93) | Thermo Fisher Scientific | Cat#: 12-0161-83 |
| CD150-PE (Clone: TC15-12F12.2) | BioLegend | Cat#: 115904 |
| CD45R(B220)-PerCP/Cy5.5 (Clone: RA3-6B2) | Thermo Fisher Scientific | Cat#: 45-0452-1631 |
| Sca-1-PerCP/Cy5.5 (Clone: D7) | Thermo Fisher Scientific | Cat#: 45-5981-82 |
| CD19-PE/Cy7 (Clone: eBio1D3) | Thermo Fisher Scientific | Cat#: 25-0193-82 |
| CD24(HAS)-PE/Cy7 (Clone: M1/69) | BD Biosciences | Cat#: 560536 |
| CD127-PE/Cy7 (Clone: eBioSB/199) | Thermo Fisher Scientific | Cat#: 25-1273-82 |
| Ly-6G-PE/Cy7 (Clone: 1A8) | BD Biosciences | Cat#: 560601 |
| CD117(c-Kit)-APC (Clone: ACK2) | Thermo Fisher Scientific | Cat#: 17-1172-83 |
| CD43-APC (Clone: S7) | BD Biosciences | Cat#: 560663 |
| CD45R(B220)-APC (Clone: RA3-6B2) | Thermo Fisher Scientific | Cat#: 17-0452-83 |
| Rat IgG2a, к-APC (Clone: RTK2758) | BioLegend | Cat#: 400511 |
| Blimp-1-APC (Clone: 5E7) | BioLegend | Cat#: 150007 |
| Streptavidin-APC | Thermo Fisher Scientific | Cat#: 17-4317-82 |
| Streptavidin-eFluor 450 | Thermo Fisher Scientific | Cat#: 48-4317-82 |
| CD45.2-eFluor 450 (Clone: 104) | Thermo Fisher Scientific | Cat#: 48-0454-80 |
| CD138-Brilliant Violet 421 (Clone: 281-2) | BD Biosciences | Cat#: 562610 |
| CD34-Brilliant Violet 421 (Clone: SA376A4) | BioLegend | Cat#: 152208 |
| CD11b-eVolve 605 | Thermo Fisher Scientific | Cat#: 83-0112-42 |
| Streptavidin-Brilliant Violet 605 | BioLegend | Cat#: 405229 |
| IgD-Brilliant Violet 605 (Clone: 11-26c.2a) | BioLegend | Cat#: 405727 |
| CD45R(B220)-Alexa Fluor 700 (Clone: RA3-6B2) | BioLegend | Cat#: 103232 |
| CD45.1-APC-eFluor 780 (Clone: A20) | Thermo Fisher Scientific | Cat#: 47-0453-82 |
| Unlabeled anti-mouse CD138 | Laboratory of Sherie L Morrison | N/A |
| Unlabeled anti-human CD20 | Laboratory of Sherie L Morrison | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant mouse SCF | Thermo Fisher Scientific | Cat#: PMC2115 |
| Recombinant mouse Flt-3L | R&D Biosystems | Cat#: 427-FL-025/CF |
| Recombinant mouse IL-3 | Thermo Fisher Scientific | Cat#: PMC0035 |
| Recombinant mouse TPO | Thermo Fisher Scientific | Cat#: PMC1144 |
| Recombinant mouse IL-7 | Biosource International | N/A |
| Recombinant mouse IL-6 | Thermo Fisher Scientific | Cat#: PMC0065 |
| Recombinant mouse IL-11 | R&D Biosystems | Cat#: 418-ML/CF |
| Recombinant mouse GM-CSF | R&D Biosystems | Cat#: 415-ML |
| Recombinant mouse IL-1β | Thermo Fisher Scientific | Cat#: PMC0816 |
| Recombinant mouse TNF-α | R&D Biosystems | Cat#: 410-MT |
| Anakinra (Kineret) | UCLA Pharmacy | N/A |
| Enbrel (Etanercept) | UCLA Pharmacy | N/A |
| MethoCult H4100 | STEMCELL Technologies | Cat#: 04100 |
| 4% Paraformaldehyde | Alfa Aesar | Cat#: J61899 |
| BD Perm/Wash | BD Biosciences | Cat#: 51-2091KZ |
| True-Nuclear Transcription Factor Buffer Set | BioLegend | Cat#: 424401 |
| TRIzol LS Reagent | Thermo Fisher Scientific | Cat#: 10296028 |
| LPS from Escherichia coli O55:B5 | Sigma-Aldrich | Cat#: L6529-1MG |
| Critical Commercial Assays | ||
| RNeasy Plus Micro Kit | Qiagen | Cat#: 74034 |
| RNeasy Micro Kit | Qiagen | Cat#: 74004 |
| RT2 First Strand Kit | Qiagen | Cat#: 330404 |
| RT2 Profiler PCR Array: Mouse Inflammatory Response and Autoimmunity | Qiagen | Cat#: PAMM-077Z |
| Custom RT2 Profiler PCR Array: | Qiagen | Cat#: CLAM30623 |
| TaqMan Universal PCR Master Mix, No AmpErase UNG | Thermo Fisher Scientific | Cat#: 4324018 |
| iQ SYBR Green Supermix | Bio-Rad | Cat#: 170-8882 |
| SMARTer Stranded Total RNA-seq (Pico) Kit | Clontech | Cat#: 634411 |
| PROTOCOL Hema 3 manual staining system | Fisher Scientific | Cat#: 23-123869 |
| Deposited Data | ||
| RNA-Seq of Young and Old PCs | This paper | GEO: GSE112939 |
| RNA-Seq of Young and Old My-HSCs | Montecino-Rodriguez et al., 2019 | GEO: GSE112769 |
| RNA-Seq of In Vitro and In Vivo Derived B Cell and PC Subsets | Shi et al., 2015 | GEO: GSE60927 |
| RNA-Seq of PC Subsets | Lam et al., 2018 | GEO: GSE115858 |
| Experimental Models: Cell Lines | ||
| GFP-expressing OP9 Stromal Cells | Schmitt and Zuniga-Pflucker, 2002 | N/A |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J Mice (Young and Middle-aged) | UCLA DLAM | N/A |
| C57BL/6J Mice (Young) | The Jackson Laboratory | JAX: 000664 |
| C57BL/6J Mice (Old) | National Institute on Aging (NIA) | N/A |
| B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) Mice (Young) | The Jackson Laboratory | JAX: 002014 |
| Oligonucleotides | ||
| Gapdh (Mm99999915_g1) | Thermo Fisher Scientific | Cat#: 4331182 |
| Tlr4 (Mm00445273_m1) | Thermo Fisher Scientific | Cat#: 4331182 |
| Tnf (Mm01161290_g1) | Thermo Fisher Scientific | Cat#: 4331182 |
| Il1b (Mm00434228_m1) | Thermo Fisher Scientific | Cat#: 4331182 |
| Il6 (Mm00446190_m1) | Thermo Fisher Scientific | Cat#: 4331182 |
| Ccl3 (Mm00441259_g1) | Thermo Fisher Scientific | Cat#: 4331182 |
| Cxcl10 (Mm00445235_m1) | Thermo Fisher Scientific | Cat#: 4331182 |
| Software and Algorithms | ||
| FlowJo (v10) | FlowJo | https://www.flowjo.com/ |
| Graphpad Prism (v5) | Graphpad |
https://www.graphpad.com/ scientific-software/prism/ |
| bcl2fastq2 (v2.17) | Illumina |
https://support.illumina.com/downloads/ bcl2fastq-conversion-software-v2-20.html |
| STAR ultrafast universal RNA-seq aligner v2.5.2b | Dobin et al., 2013 | N/A |
| RStudio | RStudio | https://www.rstudio.com/ |
| Metascape | Tripathi et al., 2015 |
http://metascape.org/gp/ index.html#/main/step1 |
| Cytoscape | Shannon et al., 2003 | http://www.cytoscape.org/ |
| SaVanT | Lopez et al., 2017 | N/A |
| Qiagen Data Analysis Center | Online |
https://www.qiagen.com/us/shop/ genes-and-pathways/data-analysis- center-overview-page/?akamai-feo=off |