Table 2.
Null tumorigenicity in HPE-15 cellsa as determined by xenograft tumor formation.
| Subject mouse strains | Group size | Route of inoculation | Cell numbers per site | Total number of sites | Matrigel co-injectionb | Number of tumors formedc |
|---|---|---|---|---|---|---|
| NCrnu/nu | 5 | s.c. | 2 × 106 | 10 | 0 | |
| 5 | s.c. | 5 × 106 | 10 | 0 | ||
| 6 | i.f | 2 × 106 | 12 | 0 | ||
| SCID | 5 | s.c. | 2 × 106 | 10 | Yes | 0 |
| 5 | s.c. | 5 × 106 | 10 | Yes | 0 | |
| 5 | s.c. | 2 × 107 | 10 | Yes | 0 | |
| NSG | 5 | s.c. | 2 × 106 | 10 | Yes | 0 |
| 5 | s.c. | 5 × 106 | 10 | Yes | 0 |
a.
Cells between passages 35 and 45 were used in the assay.
b.
Cells pre-mixed with 50% Matrigel were used in the inoculation.
c.
Xenograft tumor formation was determined 12 months after the inoculation.