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. Author manuscript; available in PMC: 2020 Aug 20.
Published in final edited form as: Immunity. 2019 Jul 23;51(2):258–271.e5. doi: 10.1016/j.immuni.2019.06.015

Figure 4. Setdb2 Is Significantly Reduced in Diabetic Wound Macrophages.

Figure 4.

(A) Setdb2 expression in macrophages from wounds of DIO and control mice at day 5 post-injury (n = 10 per group).

(B) SETDB2 protein in wounds from DIO and control mice on days 0 and 5 post-injury via western blot (n = 10 mice per group). Immunohistochemistry was performed for SETDB2 in DIO and control wounds. Representative slides are shown.

(C) ChIP analysis of Setdb2 and H3K9me3 at the IL1β, TNFα and NOS2 promoters of wound macrophages from DIO and control mice at day 5 post-injury (n = 12 mice per group).

*p < 0.05, **p < 0.01. All data represent 3 independent experiments. Data are presented as the mean ± SEM. Data were first analyzed for normal distribution, and if data passed the normality test, two-tailed Student’s t test was used. For comparison among multiple groups, ANOVA followed by Newman-Keuls post hoc test was used.