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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Mol Microbiol. 2019 May 22;112(2):532–551. doi: 10.1111/mmi.14273

Figure 10: Strains lacking ΔcspA or ΔyjbH have differing profiles of the σB-dependent asp23 promoter activity.

Figure 10:

(A) Fluorescence and cell density (OD650) were simultaneously measured in S. aureus wildtype (closed circle), ΔcspA (open diamond), ΔyjbH (lighter closed square) and ΔsigB mutant (open triangle) strains and their chromosomally complemented equivalents (cspA+, closed diamond, yjbH+, darker closed square) carrying the plasmid pALC2201 (asp23 promoter driving gfp) during 16 hours of growth in TSB. GFP fluorescence was then normalized to OD650, with each value representing the mean of two biological replicates that were read in triplicate.

(B) Growth curve of SH1000 wildtype and mutant strains. Cell densities of S. aureus wildtype (closed circle), ΔcspA (open diamond), ΔyjbH (lighter square) and ΔsigB mutant (open triangle) strains and their chromosomally complemented equivalents (cspA+, closed diamond, yjbH+, darker square) carrying the plasmid pALC2201 were measured at OD650, with each value representing the mean of two biological replicates that were read in triplicate.