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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Mol Microbiol. 2019 May 22;112(2):532–551. doi: 10.1111/mmi.14273

Figure 6: Expression of cspA in trans cross-complements carotenoid production in a SH1000 ΔyjbH strain.

Figure 6:

Carotenoid was extracted from two biological replicates each of S. aureus SH1000 wildtype, ΔcspA, ΔyjbH and ΔsigB mutant strains with either pEPSA5, pALC7091 (pEPSA5::yjbH) or pALC7342 (pEPSA5::cspA) that were grown overnight on solid Tryptic Soy agar. The carotenoid levels were normalized to cell density and compared as a percentage of wildtype set at 100%. The experiments were repeated three times, with representative percentages from one experiment displayed. The asterisks indicate statistical significance between wildtype and mutant strains, determined using Student t-test (**, P < 0.005; ***, P <0.0005).