TABLE 1:

Strain	Genotype	Source or reference
S. aureus
BD1	COL with aphA inserted upstream of cspB	(Duval et al., 2010)
JE2	USA300 LAC derivative cured of its plasmids	(Fey et al., 2012)
NE896	JE2 yjbH::tn	(Fey et al., 2012)
SH1000	8325–4 strain with repaired rsbU gene	(Horsburgh et al., 2002)
SK12	SH1000 with Tn551 insertion upstream of cspA	(Katzif et al., 2003)
Δspx	AR738, 8325–4 Δspx	(Pamp et al., 2006)
spx-c	8325–4 spx mutant with a native chromosomal copy of spx	(Pamp et al., 2006)
ALC3085	SH1000 sigB::erm	(Schmidt et al., 2004)
ALC5105	SH1000 ΔclpP	(Donegan et al., 2010)
ALC7137	SH1000 ΔyjbH	(Donegan et al., 2014)
ALC8112	SH1000 ΔcspA	This work
ALC8121	SH1000 yjbH mutant with a native chromosomal copy of yjbH	This work
ALC8212	SH1000 cspA mutant with a native chromosomal copy of cspA	This work
ALC8249	SH1000 ΔcspC	This work
ALC8581	SH1000 cspB::aphA	This work
ALC8702	MRSA JE2	(Fey et al., 2012)
ALC8704	JE2 with spx C10A mutation	Vanai Thomas, Univ. of Nebraska Medical School
ALC8706	JE2 with spx C10A mutation chromosomally repaired	Vanai Thomas, Univ. of Nebraska Medical School
ALC8971	ALC8702 with pALC8135 (pALC1484 with cspA promoter driving gfpuvr)	This work
ALC8972	ALC8704 with pALC8135	This work
ALC8973	ALC8706 with pALC8135	This work
ALC8974	ALC8702 with pEPSA5 containing a xylose-inducible promoter	This work
ALC8975	ALC8702 with pALC7342 (pEPSA5::cspA)	This work
ALC8976	ALC8704 with pEPSA5	This work
ALC8977	ALC8704 with pALC7342	This work
E. coli
XL-1 Blue	General cloning strain	Agilent
BL21 (DE3) pLysS	General expression strain	(Studier et al., 1990)
ALC7884	DC10B with pCR2.1-AmpS::hsdMS-2CC8	(Jones et al., 2015)
ALC7885	DC10B with pACYC184::hsdMS-2CC8	(Jones et al., 2015)
Plasmid	Characteristics	Source or reference
pMAD	E. coli/S. aureus shuttle plasmid with the ori pE194ts; bgaB Ampr Ermr	(Arnaud et al., 2004)
pET28a	E. coli expression vector	EMD Millipore
pEPSA5	Xylose-inducible shuttle vector; Ampr Cmr	(Forsyth et al., 2002)
pSK236	S. aureus/E. coli shuttle vector with pUC19 cloned into the HindIII site of pC194	(Gaskill and Khan, 1988)
pALC1484	pSK236 with a promoterless gfpuvr, a cycle-3 gfp allele.	(Kahl et al., 2000)
pALC2201	pALC1484 with asp23 promoter fragment	(Palma et al., 2006)
pALC7091	pEPSA5 with yjbH in EcoRI and BamHI sites and sarA ribosome binding site	This work
pALC7113	pEPSA5 with spx in EcoRI and BamHI sites and sarA ribosome binding site	This work
pALC7342	pEPSA5 with cspA in EcoRI and BamHI sites and sarA ribosome binding site	This work
pALC7803	pALC1484 with spx P1 promoter in EcoRI and XbaI sites preceding the gfpuvr gene	(Donegan et al., 2014)
pALC7804	pALC1484 with spx P2 promoter in EcoRI and XbaI sites preceding the gfpuvr gene	(Donegan et al., 2014)
pALC8134	pALC1484 with hbo-yjbH promoter in EcoRI and XbaI sites preceding the gfpuvr gene	This work
pALC8135	pALC1484 with cspA promoter in EcoRI and XbaI sites preceding the gfpuvr gene	This work
pALC8640	pALC1484 with msaA promoter in EcoRI and XbaI sites preceding the gfpuvr gene	This work
pALC8681	pET28a with spx and N-terminal hexahistidine fusion.	This work
pALC8682	pET28a with αCTD of S. aureus and N-terminal hexahistidine fusion.	This work