Fig. 2. Downregulation of LINC00665 inhibits hepatocellular carcinoma proliferation and induces apoptosis and autophagy in vitro. HepG2 and Huh-7 cells were transfected with siLINC00665 or siNC. (A) LINC00665 expression was detected in HepG2 and Huh-7 cells by qRT-PCR. (B) MTT assay was performed to evaluate cell proliferation. (C) Cell apoptotic rate was detected by flow cytometry. (D) Autophagy formation was analyzed by transfection of GFP-LC3 into HepG2 and Huh-7 cells. (E) Western blot was performed to detect the protein expression of Beclin-1 and LC3. ^*p<0.05. LINC00665, long intergenic non-protein coding RNA 665.