Fig. 3. LINC00665 interacts with miR-186-5p. (A) The binding sites between miR-186-5p and LINC00665 were predicted by starBase v2.0, and the luciferase reporter plasmids containing the wild-type (wt) or mutated (mut) LINC00665 binding sites of miR-186-5p were established. (B) The luciferase activity was measured in Huh-7 cells co-transfected with LINC00665-wt or LINC00665-mut luciferase reporter and miR-186-5p mimic or miR-NC. (C) The RIP assay was performed, and expression of LINC00665 was detected in samples bound to the Ago2 antibody or IgG in Huh-7 cells. (D) Detection of LINC00665 expression levels using qRT-PCR in samples pulled down by biotinylated miR-186-5p or negative control. (E) Expression levels of miR-186-5p in Huh-7 cells transfected with siLINC00665, siNC, pcDNA LINC00665, or pcDNA3.0 vector. *p<0.05. LINC00665, long intergenic non-protein coding RNA 665.
