Fig. 4. LINC00665 attenuates the tumor-suppressive effect of miR-186-5p in HCC cells in vitro. (A and B) The expression levels of miR-186-5p were measured in HCC tissues and cell lines by qRT-PCR. (C–G) HepG2 and Huh-7 cells were transfected with miR-NC, miR-186-5p mimic, pcDNA3.0 vector+miR-186-5p mimic, and pcDNA LINC00665+miR-186-5p mimic. (C) miR-186-5p expression was detected in HepG2 and Huh-7 cells by qRT-PCR. (D) Cell proliferation was detected by MTT assay. (E) Cell apoptotic rate was analyzed by flow cytometry. (F) Autophagy formation was analyzed by transfection of GFP-LC3 into HepG2 and Huh-7 cells. (G) Western blot was performed to detect the protein expression of Beclin-1 and LC3. ^*p<0.05. LINC00665, long intergenic non-protein coding RNA 665; HCC, hepatocellular carcinoma.