Fig. 5. MAP4K3 a target gene of miR-186-5p. (A) The binding sites between miR-186-5p and MAP4K3 were predicted by TargetScan, and the luciferase reporter plasmids containing the wild-type (wt) or mutated (mut) MAP4K3 binding sites of miR-186-5p were established. (B) The luciferase activity was measured in Huh-7 cells co-transfected with MAP4K3-wt or MAP4K3-mut luciferase reporter and miR-186-5p mimic or miR-NC. (C) The RIP assay was performed, and expression of MAP4K3 was detected in the samples bound to the Ago2 antibody or IgG in Huh-7 cells transfected with miR-186-5p mimic or miR-NC. (D) Detection of MAP4K3 expression level using qRT-PCR in Huh-7 cells transfected with NC mimic, miR-186-5p mimic, NC inhibitor, or miR-186-5p inhibitor. ^*p<0.05. LINC00665, long intergenic non-protein coding RNA 665.