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. 2019 Aug 21;9:12212. doi: 10.1038/s41598-019-48589-0

Figure 3.

Figure 3

GATA2 knockdown impairs leukemia growth/survival of HL60 and K562 cells but does not affect NOMO1 cells. HL60, K562 and NOMO1 cells were transduced with lentiviruses encoding a short hairpin against human GATA2 (or scramble control) and a GFP reporter. GFP+ cells were FAC-sorted and cultured for 72 hours. (A) Number of live HL60 cells 72 hours after sorting (n = 4). (B) Frequency of apoptotic HL60 cells (annexin V+) 72 hours after sorting (n = 4). (C) Number of live K562 cells 72 hours after sorting (n = 3–4). (D) Frequency of apoptotic K562 cells (annexin V+) 72 hours after sorting (n = 4). (E) Number of live NOMO1 cells 72 hours after sorting (n = 3–4). (F) Frequency of apoptotic NOMO1 cells (annexin V+) 72 hours after sorting (n = 4). Data are mean ± SEM. Statistical analysis: Mann-Whitney test.