Fig. 3.
CNO prevents the fluoxetine-induced increase in activity of dentate gyrus neurons in vivo. a Representative images of HA-Tag + Egr1 + double-labeled dentate gyrus cells. b Quantification of HA-Tag + Egr1 + cells in the dentate gyrus (by two-way ANOVA: Fluoxetine F1,16 = 4.288, P = 0.0549; CNO F1,16 = 26.16, ***P = 0.0001; Interaction F1,16 = 1.184, P = 0.293). c Representative images of c-Fos-positive dentate gyrus cells. d Quantification of c-Fos expression in the dentate gyrus. (by three-way ANOVA: Fluoxetine F1,32 = 21.55, ***P < 0.0001; hM4Di F1,32 = 9.01, **P = 0.005; CNO F1,32 = 10.85, **P = 0.002; hM4Di*CNO F1,32 = 6.14, *P = 0.019; Tukey posthoc test + hM4Di + CNO Saline treatment versus other saline treatment groups *P < 0.05; + hM4Di + CNO Fluoxetine treatment versus other fluoxetine treatment groups *P < 0.05). Data are presented as means ± s.e.m. Scale bars 50 μm