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. 2019 Aug 21;9:12143. doi: 10.1038/s41598-019-48613-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Measurements of choroidal thickness and vascularity. (A) Nasal parafoveal choroidal thickness (CT1), subfoveal choroidal thickness (CT2), and temporal parafoveal choroidal thickness (CT3) were obtained using enhanced depth image optical coherence tomography. CT was measured as the distance between Bruch’s membrane and the choroid-scleral interface. (B) The subfoveal choroidal area with a width of 3 mm, centered at the fovea, was selected. Using ImageJ software, the image was binarized with Niblack’s method, and the ratio of vascular area (black pixels) to stromal area (white pixels) was quantified.