Fig. 3.

Isolation and characterization of plant NVs. a Schematic representation of plant NVs isolation of Juglans regia, Juglans californica, Corylus avellana, Bertholletia excelsa, and Malus domestica. b Representative images obtained through transmission electron microscopy (left panels) and intensity-weighted size distribution calculated by dynamic light scattering (right panels) of NVs isolated from J. californica, J. regia, and C. avellana. Scale bars: 200 nm. c Isolated NVs were stained with an RNA-specific probe and the percentage of positive NVs was calculated by cytofluorimetry. The histogram reported is from one experiment representative of three giving similar results