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. 2019 Aug 21;9:12206. doi: 10.1038/s41598-019-48432-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PDE5 and NOTCH3 expression during development of mouse and human fetal aortas. Representative immunohistochemical evaluation of PDE5 and NOTCH3 on early mouse (12 days post coitum, dpc) and human (7 weeks post-conception, wpc) embryos showing positivity for both protein in the medial layer of the dorsal aorta, pulmonary arteries, gastrointestinal tract, and in megakaryoblasts of the liver in both mouse and human embryos. Black arrows indicate thoracic aorta, abdominal aorta, celiac artery, pulmonary vein and pulmonary artery respectively.