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. 2019 Aug 21;9:12191. doi: 10.1038/s41598-019-48400-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of actin binding proteins at the microridge at 48 hpf. Immunolocalisation of ArpC2 (b), WASL (e), and Cofilin (h) at 48 hpf with LifeAct-RFP in magenta that marks F-actin (a,d,g) at 48 hpf. Respective merges are shown in (c,f,i). Plasmid encoded Eplin-β (j), and Filamin A (m) in green localize to microridges marked with phalloidin (k,n) in magenta in respective merges (l,o). Plasmids were injected at the 1 cell stage and expressed in a clonal fashion. While all other images are obtained from the head epidermis, Eplin images are taken on the flank. Scale bars are 10 µm.