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. 2019 Aug 21;9:12221. doi: 10.1038/s41598-019-48759-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GAS5 suppresses microglial M2 polarization and promotes M1 polarization after OGD/R. RT-PCR analysis of M1 (MCP-1, iNOS, CD32) and M2 (Ym1/2, Arg-1, CD206) markers in BV2 microglial cells transduced with the si-GAS5/siRNA-NC (A) or pcDNA3.1-GAS5/pcDNA3.1-NC (B). (C,D) ELISA analysis of TNF-α, and IL-10 in culture supernatants of BV2 microglial cells transduced with the si-GAS5/siRNA-NC or pcDNA3.1-GAS5/pcDNA3.1-NC. (E,G) Western blotting analysis of CD16/32 and CD206 in microglia transduced with the si-GAS5/siRNA-NC. (F,H) Western blotting analysis of CD16/32 and CD206 in microglia transduced with the pcDNA3.1-GAS5/pcDNA3.1-NC. Data are represented as mean ± SD, ^*P < 0.05, ^**P < 0.01 versus Control; ^#P < 0.05, ^##P < 0.01.