Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 21;9:12207. doi: 10.1038/s41598-019-48226-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Examples of analysis techniques for collected biospecimens. (a) Hematoxylin & Eosin staining of HS lesional skin from fresh-frozen tissue. (b) Fontana-Masson staining of HS skin from paraffin-embedded tissue. Scale bar: 100 μm. (c) Immunofluorescence of HS skin for keratin 14 (red), MPO-myeloperoxidase (green), and DAPI (blue). (d) HS skin was digested and primary fibroblasts (first panel) and primary epidermal cells- keratinocytes and melanocytes- (second panel) were cultured. Peripheral blood was collected from HS patients and monocytes/macrophages (third panel) and neutrophils (fourth panel) were isolated and cultured. Scale bar: 50 μm. (e) HS skin was digested into single cell suspension and stained for flow cytometry analysis. (f) Protein was extracted from peripheral blood cells and used to perform Western blot. Uncropped Western Blot in Supplemental Fig. S1. (g) RNA was extracted from homogenized healthy control and HS skin and was quantified with a spectrophotometer. The 260/280 absorbance ratio of ~2 represents the purity and quality of the RNA. (h) Heatmap representing the microbiome of healthy control and HS skin developed by next-generation sequencing.