Table 2.

Recommendations for tissue and biospecimen collection.

Recommendations	Overview
Consents and Protocols	IRB approved consents and protocols Tissue and biospecimens are collected and utilized for treatment recommendations and research
Tissue collection & processing	Collect → Observe → Proper storage Transport from OR to bench Proper storage of lysate, RNA, OCT, and paraffin samples Cryostat sectioning of tissue for IHC and IF
Preparation of tissue for protein analysis	Homogenize whole tissue for Western Blot, ELISA, proteomics, etc.
Preparation of tissue for nucleic acid analysis	DNA/RNA extraction for genetic sequencing and analysis
Preparation of tissue for cell culture- Primary and immune cell extraction	Extraction of primary cells Cell culture of fibroblasts, keratinocytes, and melanocytes Extraction of immune cells
Processing of saliva samples	DNA sample preparation
Blood processing for serum, nucleic acid and immune cell isolation	Collection and separation, isolation, flow cytometry, RNA extraction
Skin swabs for microbiome studies	Sequencing and analysis for evaluation of microbial composition
Overview of sample storage	−20 °C, −80 °C, liquid nitrogen options for storage