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. 2019 Aug 21;9:12211. doi: 10.1038/s41598-019-48659-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spectral analysis of the decellularization process using the fiber optic spectrophotometer. (a) Schematic diagram of the setup of spectral measurements. (b) Normalized intensities (0–1) of the reflectance spectra versus wavelength obtained during the perfusion with SDS and the LED spectrum. (c) Absolute intensities of reflectance spectra demonstrating maximum values at between 1 and 2 hours. (d) Absolute intensities of transmittance spectra showing an overall increase over time. (e) Normalized (0–1) spectra of reflectance at the initial moment of SDS perfusion, and transmittance at 30 minutes of SDS perfusion, compared with the light source profile (LED), evidencing a band shift at 600 nm.