Fig. 2. Generation of chronic iron-exposed and transformed FTSECs.

a Schematic summarizing the maintenance of FT194 cells in FAC. b Schematic depicting the method for transforming FT194 cells via retroviral infection of H-Ras^V12A, c-Myc^T58A, and SV40 LTAg. c Representative light microscope images of FT194-untreated and 250 nM FAC-treated cells (p = 19). Images were captured at ×100 magnification and a subpanel of the image was enlarged in PowerPoint. d Representative light microscope images of FT194 control virus (CV) and oncogenic cocktail virus (OCV) cells (p = RV + 3). Images were captured at ×100 magnification and a subpanel of the image was enlarged in PowerPoint