Table 1.
List of primary and secondary antibodies
| Primary antibodies | Host IgG | Dilution | HIER | Producer | Code number |
|---|---|---|---|---|---|
| AQP4 | Rabbit IgG | 1:3000–1:6000 | M6 | AVIVA | OABB01958 |
| S100 | Mouse IgG2a | 1:2000 | M6 | Abcam | ab14849 |
| S100 | Rabbit IgG | 1:250 (F), 1:3000–1:6000 | – | Dako | Z0311 |
| GFAP | Mouse IgG1k | 1:50–1:200 | TEG/M6 | Dako | M0761 |
| GFAP | Rabbit IgG | 1:10000 | TEG | Dako | Z0334 |
| YKL‐40 | Mouse IgG2kb | 1:50/1:100 | – | * | 201.F9 |
| C3d | Rabbit IgG | 1:200–1:600 | M6 | Dako | A0063 |
| Secondary antibodies | Host IgG | Dilution | Target | Producer | Code number |
| Alexa Fluor 488 | Goat IgG | 1:100 | Anti‐mouse | Jackson | 115‐545‐166 |
| Alexa Fluor 594 | Goat IgG | 1:100 | Anti‐rabbit | Fisher | T20925 |
| Dylight 594 | Goat IgG | 1:100 | Anti‐mouse | Jackson | 115‐515‐146 |
| Dylight 594 Strept. | – | 1:200 | – | Vector | SA5594 |
| Biotin‐SP | Donkey IgG | 1:200 | Anti‐rabbit | Jackson | 711‐066‐152 |
F, fluorescence; HIER, heat‐induced epitope retrieval.
TEG: 1,211 g Trizma® Base (Sigma‐Aldrich T1503) + 0.190 g ethylene glycol tetraacetic acid (EGTA) buffer (Sigma‐Aldrich E‐4378) + 1000 mL distilled water (1dw) adjusted to pH 9. M6: 21.014 g citric acid monohydrate (Merck 100244) in 1dw + 29,410 g tri‐sodium citrate dihydrate (Merck 106448) in 1dw adjusted to pH 6.
Producers: Abcam, Cambridge, UK. Aviva Systems Biology, San Diego, CA, USA. Dako, Agilent, Glostrup, Denmark. Fisher Scientific, Toronto, CA. Jackson ImmunoResearch, Ely, UK. Vector Laboratories, Peterborough, UK.
*
Kind gift of Prof. Julia S. Johansen, Herlev Hospital, Copenhagen, Denmark, and Paul A. Price, UCSD, USA.