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. 2019 Aug 21;9:12204. doi: 10.1038/s41598-019-48523-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Promoter-driven luciferase activity increases in response to g-lysin treatment. Bar graphs represent the change in promoter activity of PHC19 (a), AraGT1 (b), RHM1 (c), and SEC61G (d) genes, using two independent promoter-transformed lines. Luciferase activity is expressed as relative light units (RLUs) based on luminescence quantification. Untreated gamete control samples are represented by black bars. Grey bars represent gamete cells treated with g-lysin. Error bars are one standard deviation from the mean of biological triplicate data. Welch’s t-test indicates statistical significance at p ≤ 0.05 (*), p ≤ 0.01 (**); α = 0.05. RHM1-2 and SEC61G-2 showed a large variation that resulted in significance at 0.055 and 0.064, respectively, below the 95% cutoff.