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. 2019 Aug 21;9:12204. doi: 10.1038/s41598-019-48523-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcripts are differentially affected by CHX pretreatment prior to gametolysin treatment. (a,b) Bar graphs represent the change in gene expression for cell wall protein (a) and protein processing type (b) CW gene transcripts in wild type (CC-125) cells. Untreated gamete control samples represented by black bars, −CHX −g-lysin; medium grey bars for CHX treated cells, +CHX; dark grey bars for cells treated with g-lysin, +g-lysin; light grey bars for both CHX and g-lysin-treated cells, +CHX +g-lysin. Gene expression is quantified in terms of fold change of the untreated control condition. Error bars represent one standard deviation from the mean of biological triplicate samples. Letters below gene names indicate a significant difference between the specified samples using a two-way ANOVA at p ≤ 0.01; α = 0.05; ‘a’ for −CHX −g-lysin vs. +g-lysin, ‘b’ for −CHX -g-lysin vs. +CHX, ‘c’ for −CHX -g-lysin vs. +CHX +g-lysin, and ‘d’ for +g-lysin vs. +CHX +g-lysin.