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. 2019 Aug 21;9:12204. doi: 10.1038/s41598-019-48523-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Isotonic and hypertonic conditions negatively affect the g-lysin-induced CW gene activation. (a–d) Bar graphs represent the change in gene expression by g-lysin treatment for PHC19 (a), GAS28 (b), GAS30 (c), and SEC61G (d) in wild type (CC-125) cells. g-lysin treatment was done in three constant osmotic conditions, hypo- (64 mOsm), iso- (175 mOsm), and hyper- (204 mOsm). Untreated gamete control samples represented by black bars, −g-lysin; medium grey bars for g-lysin-treated cells, +g-lysin. Gene expression is quantified in terms of fold change of the untreated control in hypotonic condition. Error bars represent one standard deviation from the mean of biological triplicate samples. Welch’s t-test indicates statistical significance at p ≤ 0.01 (*), p ≤ 0.001 (**); α = 0.05.