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. 2019 Jul 25;18(3):3195–3201. doi: 10.3892/ol.2019.10662

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Copyright: © Du et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effects of shikonin on paclitaxel-induced apoptosis. KYSE270 cells were exposed to paclitaxel (100 nM) for 24 h in either the presence or absence of shikonin (1 µM). (A) The percentage of Sub-G0 cells was calculated based on cell cycle data. Flow cytometric analysis of Annexin-V and propidium iodide-stained cells following treatment with (B) DMSO, (C) paclitaxel, (D) shikonin, and (E) paclitaxel and shikonin. (F) The percentage of apoptotic cells in each treatment group. Data were analyzed by one-way ANOVA with Dunnett's multiple comparisons test, and presented as the mean ± standard error or the mean from three independent experiments. **P<0.001. Ctr, control; PAX, paclitaxel; SHK, shikonin.