Figure 1.

Silencing ROR1 with siRNA significantly reduces the expression of ROR1 in non-small cell lung cancer cell lines. (A) NCI-H1975, PC-9erlo and XLA-07 cell lines were treated with Mock, 25 nM siROR1 or siNC for 72 h, and ROR1 expression levels were examined using flow cytometry with R12, a chimeric rabbit/human anti-ROR1 monoclonal antibody, or normal human IgG. The background signal stained with human IgG is presented in gray. (B) MFI value of ROR1 expression in NCI-H1975, PC-9erlo and XLA-07 cell lines (**P<0.01, ***P<0.001, mock vs. siROR1). Experiments were performed 3 times (n=3). The y-axis represents the number of cells acquired by flow cytometry. ROR1, receptor tyrosine kinase-like orphan receptor 1; si, small interfering; NC, negative control; Mock, complete medium; MFI, Mean fluorescence intensity.