Figure 3.

Blocking ROR1 with siRNA enhances the apoptosis-inducing role of erlotinib in non-small cell lung cancer cell lines. NCI-H1975, PC-9erlo and XLA-07 cell lines were treated with Mock, 20 nM siNC, 20 nM siROR1, erlotinib alone, erlotinib + 20 nM siNC or erlotinib + 20 nM siROR1. The concentration of erlotinib was 2.5 µM for PC-9erlo and NCI-H1975 cells, and 10 µM for XLA-07 cells. Following treatment, apoptosis of the cells was analyzed by Annexin V/propidium iodide staining. Experiments were performed 3 times (n=3). ROR1, receptor tyrosine kinase-like orphan receptor 1; si, small interfering; NC, negative control; Mock, complete medium.