Figure 5.

SKA2 regulates miR-520a-3p-mediated inhibition of SGC-7901 cells proliferation, migration and invasion. (A) After cells were transfected with a SKA2 cDNA plasmid, the expression of SKA2 was notably increased in SGC-790 cells. Following co-transfection of miR-520a-3p mimics and SKA2 cDNA into SGC-7901 cells, (B) expression of SKA2 and (C) cell viability were examined by western blotting and cell proliferation assay, respectively. (D) Migration distance and (E) number of invasive cells in GC cells following treatment with miR-520a-3p mimics and SKA2 cDNA were detected by wound healing and Transwell assays, respectively. **P<0.01, ***P<0.001 vs. the MOCK group. ×100 magnification. Scale bar, 100 µm. SKA2, spindle kinetochore-associated protein 2; miR, microRNA; GC, gastric cancer; MOCK, mock transfected cells; NC, negative control; OD, optical density.