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. 2019 Jul 25;18(3):3256–3264. doi: 10.3892/ol.2019.10664

Figure 2.

Figure 2.

MHY695 induces cytotoxicity by promoting apoptosis. (A) Morphological changes in HCT116 colon cancer cells were observed by confocal microscopy. Cells were treated with vehicle or MHY695 (25 or 50 µM) for 24 h. Apoptotic body formation (red arrows) was observed using DAPI staining (blue) in the MHY695-treated groups. The release of cytochrome C (green) was also observed in the MHY695-treated groups. (B) Apoptotic or necrotic cells were identified using flow cytometry. HCT116 cells were treated with MHY695 or vehicle for 24 h, prior to staining with Annexin V and PI. Annexin V-bound or PI-positive cells were subsequently quantified. MHY695, (Z)-5-(3-ethoxy-4-hydroxybenzylidene)-2-thioxothiazolidin-4-one; PI, propidium iodide.