Figure 3.

MHY695 modulates the expression of apoptosis-associated proteins. HCT116 cells were treated with vehicle or MHY695 (25 or 50 µM) for 24 h. Cellular proteins were separated using SDS-PAGE and transferred to PVDF membranes. The bands were detected using ECL solution and a chemiluminescence system. The bands are representative of three independent experiments. MHY695, (Z)-5-(3-ethoxy- 4-hydroxybenzylidene)-2-thioxothiazolidin-4-one; p-, phospho-; Akt, protein kinase B; Bad, Bcl-2-associated agonist of cell death; Mcl-1, induced myeloid leukaemia cell differentiation protein; PARP, poly(ADP-ribose) polymerase.