Comparison of the Recovery (%) of Eluforsen after 2-Day Incubations with Purified Nucleases
1 μg of eluforsen was incubated with either endonuclease (RNase A), exonuclease (Exo-T), or both enzymes (double-digestion) at 37°C for 2 days. A negative control was prepared by incubation of eluforsen in buffer but without any endo- and exonuclease. The recoveries of eluforsen were 100.00% ± 3.35%, 90.84% ± 12.23%, 109.17% ± 2.90%, and 105.46% ± 1.73% (mean ± standard error mean) in the buffer only, RNase A, both enzymes, and Exo-T, respectively.